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North American Mosquito
East (NAM-e) test kit

Quickly detect mosquito virus
threats

The Vector Smart™ North American Mosquito East (NAM-e) test is a real-time PCR multiplex test intended to detect West Nile virus (WNV), St. Louis encephalitis virus (SLEV), and eastern equine encephalitis virus (EEEV) through extracted ribonucleic acid (RNA) in mosquito samples.

Rigorously tested for virus detection

The Vector Smart NAM-e test kit has been tested with QIAamp Viral RNA Mini Extraction Kit (Qiagen), MagMax -96 Viral RNA Isolation Kit (Thermo Fisher Scientific), the sbeadex livestock kit (LGC), and CoWin Bio Viral DNA/RNA kit CW-E-001 or -002 (Jiangsu CoWin Biotech Co., Ltd), and run using the Co-Dx Box thermal cycler. Triton X-100 (Sigma-Aldrich) is used to inactivate the viruses.

Run your own PCR lab

Say goodbye to long wait times and take control of your abatement program by bringing testing in-house.

Simple, streamlined workflow

Get test results in 60-90 minutes instead of waiting days or weeks for results from a centralized lab. 

Controlled for accuracy

Includes both internal and positive controls on each test to ensure testing efficacy.

Easy to use

Kits are compatible with multiple platforms & sample extraction techniques

About the Vector Smart NAM-e test

The Vector Smart NAM-e test kit showed no cross-reactivity with Japanese encephalitis virus (JEV), Yellow fever virus (YFV), Zika virus (ZIKV), dengue virus (DENV) types 1-4, chikungunya (CHIKV), Western equine encephalitis (WEEV), Nhumirim virus (NHUV), Jamestown Canyon virus (JCV), La Crosse encephalitis virus (LACV), and Hepatitis C virus (HCV) through wet-test or in-silico analysis.

Each Vector Smart NAM-e test kit includes:

  • 1×500μL (100 rxns) Vector Smart NAM-e Master Mix
  • 1×500μL (100 rxns) Vector Smart NAM-e Positive Control
  • 1×500μL (100 rxns) Vector Smart NAM-e Extraction Control
  • 1×500μL (100 rxns) Negative Control

Performance of the Vector Smart NAM-e test

Application

Qualative Multiplex PCR test for detection for WNV, SLEV, and EEEV

WNV†

SLEV†

EEEV†

Limit of Detection

(per assay)

3.84×104
3.39×103
7.77×102
Sensitivity
96.9%
99.5%
99%
Specificity
99%
99%
99%
Sample type
Mosquitos
Detection details
Detection and differentiation of West Nile virus (WNV), St. Louis encephalitis virus (SLEV), and Eastern equine encephalitis virus (EEEV).
Time to detection
63-90 minutes, depending on the machine used
Thermal cycler
compatibility

Most qPCR systems capable of the following channel compatibilities:

  • FAM
  • CF560 (VIC)
  • CF610 (ROX)
  • Quasar 670 (Cy5)
Extraction kit compatibility
QIAamp® Viral RNA Mini Kit, MagMax™ – 96 Viral RNA Isolation Kit, and sbeadex livestock kit.

† Results based on 1,420 samples run on the CoDx Box™ qPCR cycler.

Request a quote

Please cross reference the Vector Smart NAM-e kit product ID (NAMe-K-001) when you contact us.

How to use the test kit

The Vector Smart NAM-e test kit is simple and easy-to-use. Get started here.