HotStaRT™ - Reduces false readings in real-time PCR
HotStaRT™ is a unique hot start for PCR and is the first to work on both RNA and DNA reactions, reducing both false positives and negatives.
HotStaRT™ adds proprietary chemical “hooks” to an oligonucleotide, causing them to stick to the binding site at low temperatures, reducing non-specific reactions.
HotStaRT™ works on both polymerase and reverse transcriptase reactions. The charts below illustrate its effectiveness at improving sensitivity and outcomes on the reverse transcriptase reaction.